What nutrients or deficiencies cause twitching

ChrisMorell asks on Twitter: "What vitamins/nutrients would you recommend for muscle twitching (eyes, legs)? Recurring issue since I was 20. Supplementing w/ magnesium g. ~350mg per day, plenty of D, K2, E & A. Did Genova ion panel last year but maybe time for another testing round?" and Kori Dunlap asks on YouTube: "I''m low on B2 according to my NutrEval results so I'm just taking double of a B complex. However when you mentioned eye twitching my Sons test came back low B6, so he is taking that from Thorne, but this is going in the 3rd week and now I'm noticing eye twitching. Please advise. Thank you so much for your valued information!!🙏" Disclaimer: This is educational in nature. I am not a doctor and this is not medical advice. Condensed answer (see video for details): Twitching could involve excitatory or inhibitory amino acids (especially glutamate, glycine, GABA, and maybe acetylcholine), electrolytes (sodium, potassium, chloride, magnesium, and calcium), acid-base balance (beta-alanine, carnosine, and bicarbonate can be relevant), B vitamins (especially especially thiamin, especially niacin, riboflavin, and pantothenic acid, but also biotin, folate, and B12), and anything that impacts energy metabolism (caffeine, exercise, exertion, stress, hyperthyroid, hypothyroid, diabetes, inborn errors of metabolism, etc). Here are the cliff notes: https://chris-masterjohn-phd.myshopify.com/products/the-vitamins-and-minerals-101-cliff-notes And here is Testing Nutritional Status: The Ultimate Cheat Sheet: https://chris-masterjohn-phd.myshopify.com/products/testing-nutritional-status-the-ultimate-cheat-sheet

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